ISDA: 6PUA

Deposition Date 2019-07-18

Release Date 2019-09-25

Last Version Date 2023-10-11

Entry Detail

PDB ID:

6PUA

Keywords:

TRANSFERASE

Title:

The 2.0 A Crystal Structure of the Type B Chloramphenicol Acetyltransferase from Vibrio cholerae

Biological Source:

Source Organism(s):

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (Taxon ID: 243277)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.00 Å

R-Value Free:

0.18

R-Value Work:

0.16

R-Value Observed:

0.16

Space Group:

P 31 2 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Chloramphenicol acetyltransfe
Gene (Uniprot):VC_A0300
Chain IDs:A, B, C
Chain Length:212
Number of Molecules:3
Biological Source:Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

UniProt ID:Q9KMN1 Pfam ID:PF00132

Ligand Molecules

EDO

MPD

PO4

Primary Citation

Structural genomics of bacterial drug targets: Application of a high-throughput pipeline to solve 58 protein structures from pathogenic and related bacteria.

Inniss, N.L, Minasov, G, Chang, C, Tan, K, Kim, Y, Maltseva, N, Stogios, P, Filippova, E, Michalska, K, Osipiuk, J, Jaroszewki, L, Godzik, A, Savchenko, A, Joachimiak, A, Anderson, W.F, Satchell, K.J.F.Show

Microbiol Resour Announc ? e0020025 e0020025 (2025)

PMID: 40391899 DOI: 10.1128/mra.00200-25

Abstact

Antibiotic resistance remains a leading cause of severe infections worldwide. Small changes in protein sequence can impact antibiotic efficacy. Here, we report deposition of 58 X-ray crystal structures of bacterial proteins that are known targets for antibiotics, which expands knowledge of structural variation to support future antibiotic discovery or modifications.

Legend

Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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