ISDA: 6C8Q

Deposition Date 2018-01-25

Release Date 2018-02-14

Last Version Date 2023-10-04

Entry Detail

PDB ID:

6C8Q

Keywords:

TRANSFERASE

Title:

Crystal structure of NAD synthetase (NadE) from Enterococcus faecalis in complex with NAD+

Biological Source:

Source Organism(s):

Enterococcus faecalis V583 (Taxon ID: 226185)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.58 Å

R-Value Free:

0.23

R-Value Work:

0.18

R-Value Observed:

0.18

Space Group:

P 1 21 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:NH(3)-dependent NAD(+) synthe
Gene (Uniprot):nadE
Chain IDs:A, B, C, D, E, F, G, H
Chain Length:275
Number of Molecules:8
Biological Source:Enterococcus faecalis V583

UniProt ID:Q830Y9 Pfam ID:PF02540 EC:6.3.1.5

Ligand Molecules

NAD

Primary Citation

Structural genomics of bacterial drug targets: Application of a high-throughput pipeline to solve 58 protein structures from pathogenic and related bacteria.

Inniss, N.L, Minasov, G, Chang, C, Tan, K, Kim, Y, Maltseva, N, Stogios, P, Filippova, E, Michalska, K, Osipiuk, J, Jaroszewki, L, Godzik, A, Savchenko, A, Joachimiak, A, Anderson, W.F, Satchell, K.J.F.Show

Microbiol Resour Announc ? e0020025 e0020025 (2025)

PMID: 40391899 DOI: 10.1128/mra.00200-25

Abstact

Antibiotic resistance remains a leading cause of severe infections worldwide. Small changes in protein sequence can impact antibiotic efficacy. Here, we report deposition of 58 X-ray crystal structures of bacterial proteins that are known targets for antibiotics, which expands knowledge of structural variation to support future antibiotic discovery or modifications.

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Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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