ISDA: 5TXU

Deposition Date 2016-11-17

Release Date 2016-12-14

Last Version Date 2026-04-01

Entry Detail

PDB ID:

5TXU

Keywords:

HYDROLASE

Title:

1.95 Angstrom Resolution Crystal Structure of Stage II Sporulation Protein D (SpoIID) from Clostridium difficile in Apo Conformation

Biological Source:

Source Organism(s):

Peptoclostridium difficile (strain 630) (Taxon ID: 272563)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.95 Å

R-Value Free:

0.18

R-Value Work:

0.15

R-Value Observed:

0.16

Space Group:

P 31 2 1

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Stage II sporulation protein
Gene (Uniprot):spoIID
Chain IDs:A
Chain Length:354
Number of Molecules:1
Biological Source:Peptoclostridium difficile (strain 630)

UniProt ID:Q18CL6 Pfam ID:PF08486

Ligand Molecules

DMS

FMT

Primary Citation

A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile.

Rosas-Lemus, M, Dey, S, Minasov, G, Tan, K, Anderson, S.M, Brunzelle, J, Nocadello, S, Shabalin, I, Filippova, E, Halavaty, A, Kim, Y, Maltseva, N, Osipiuk, J, Minor, W, Joachimiak, A, Savchenko, A, Anderson, W.F, Satchell, K.J.F.Show

Microbiol Resour Announc 12 e0050723 e0050723 (2023)

PMID: 37747257 DOI: 10.1128/MRA.00507-23

Abstact

Clostridioides difficile causes life-threatening gastrointestinal infections. It is a high-risk pathogen due to a lack of effective treatments, antimicrobial resistance, and a poorly conserved genomic core. Herein, we report 30 X-ray structures from a structure genomics pipeline spanning 13 years, representing 10.2% of the X-ray structures for this important pathogen.

Legend

Protein

Chemical

Disease

Primary Citation of related structures

Abstact

Feedback Form