ISDA: 5A4O

Deposition Date 2015-06-11

Release Date 2016-04-06

Last Version Date 2024-05-01

Entry Detail

PDB ID:

5A4O

Keywords:

DNA BINDING PROTEIN

Title:

Crystal structure of BPSL1147, a PC4 homolog from Burkholderia pseudomallei K96243 (orthorhombic crystal form)

Biological Source:

Source Organism(s):

BURKHOLDERIA PSEUDOMALLEI (Taxon ID: 272560)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.76 Å

R-Value Free:

0.24

R-Value Work:

0.19

R-Value Observed:

0.19

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:BPSL1147
Gene (Uniprot):BPSL1147
Chain IDs:A, B
Chain Length:75
Number of Molecules:2
Biological Source:BURKHOLDERIA PSEUDOMALLEI

UniProt ID:Q63VU8 Pfam ID:PF02229

Ligand Molecules

Primary Citation

Structural Analysis and Knock-Out of a Burkholderia Pseudomallei Homolog of the Eukaryotic Transcription Coactivator Pc4.

Werten, S, Kohler, C, Bayer, N, Steinmetz, I, Hinrichs, W.Show

Gene 557 140 ? (2016)

PMID: 26625975 DOI: 10.1016/J.GENE.2015.11.037

Abstact

Homologs of the eukaryotic transcription coactivator PC4, which also functions in DNA repair and oxidative stress, were recently identified in prokaryotes. Crystallographic analysis of BPSL1147, a putative homolog from the pathogen Burkholderia pseudomallei K96243, reveals a highly conserved core structure and suggests a nucleic acid binding mode similar to that of PC4. Knock-out and complementation experiments do not reveal distinguishing phenotypes under normal growth conditions or in the presence of H2O2, arguing against a critical role in repair or the oxidative stress response of Burkholderia. These results may reflect redundancy or point at a bacteriophage origin of Burkholderia PC4 homologs.

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Primary Citation of related structures

Abstact

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