4YX7 image
Deposition Date 2015-03-22
Release Date 2015-06-03
Last Version Date 2023-09-27
Entry Detail
PDB ID:
4YX7
Keywords:
PROTEIN TRANSPORT
Title:
Complex of SpaO(SPOA1,2) and OrgB(APAR)::T4lysozyme fusion protein
Biological Source:
Source Organism(s):
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (Taxon ID: 99287)
Enterobacteria phage T4 (Taxon ID: 10665)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
2.00 Å
R-Value Free:
0.20
R-Value Work:
0.15
R-Value Observed:
0.15
Space Group:
P 1 21 1
4YX7 validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Surface presentation of antig
Gene (Uniprot):spaO
Chain IDs:A, D
Chain Length:73
Number of Molecules:2
Biological Source:Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
UniProt ID:P40699 Pfam ID:PF01052, PF26304
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Surface presentation of antig
Gene (Uniprot):spaO
Chain IDs:B, E
Chain Length:70
Number of Molecules:2
Biological Source:Salmonella typhimurium
UniProt ID:P40699 Pfam ID:PF01052, PF26304
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Oxygen-regulated invasion pro
Gene (Uniprot):E, sctL1
Mutagens:D20N,C54T,C97A,D20N,C54T,C97A
Chain IDs:C, F
Chain Length:197
Number of Molecules:2
Biological Source:Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720), Enterobacteria phage T4
UniProt ID:P0CL45,P00720 Pfam ID:PF00959 EC:3.2.1.17
Primary Citation
A common assembly module in injectisome and flagellar type III secretion sorting platforms.
Nat Commun 6 7125 7125 (2015)
PMID: 25994170 DOI: 10.1038/ncomms8125

Abstact

Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.

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Primary Citation of related structures
4YX7
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