4UQG image
Deposition Date 2014-06-23
Release Date 2014-12-03
Last Version Date 2024-01-10
Entry Detail
PDB ID:
4UQG
Keywords:
TRANSFERASE/DNA
Title:
A new bio-isosteric base pair based on reversible bonding
Biological Source:
Source Organism(s):
GEOBACILLUS STEAROTHERMOPHILUS (Taxon ID: 1422)
synthetic construct (Taxon ID: 32630)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
2.00 Å
R-Value Free:
0.21
R-Value Work:
0.17
R-Value Observed:
0.17
Space Group:
P 21 21 21
4UQG validation report
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:DNA POLYMERASE
Gene (Uniprot):polA
Chain IDs:A
Chain Length:579
Number of Molecules:1
Biological Source:GEOBACILLUS STEAROTHERMOPHILUS
UniProt ID:E1C9K5 Pfam ID:PF22619, PF00476 EC:2.7.7.49
Polymer Type:polydeoxyribonucleotide
Molecule:5'-D(*GP*AP*CP*C T0TP*TP*CP*C
Chain IDs:B
Chain Length:10
Number of Molecules:1
Biological Source:synthetic construct
Polymer Type:polydeoxyribonucleotide
Molecule:5'-D(*AP*GP*GP*GP*A SAYP*GP*G
Chain IDs:C
Chain Length:10
Number of Molecules:1
Biological Source:synthetic construct
Ligand Molecules
SO4
Peptide-like Molecules
PRD_900003
Primary Citation
"Post-it" type connected DNA created with a reversible covalent cross-link.
Angew. Chem. Int. Ed. Engl. 54 796 800 (2015)
PMID: 25446281 DOI: 10.1002/anie.201407854

Abstact

We report the development of a new heterobase that is held together through reversible bonding. The so-formed cross-link adds strong stabilization to the DNA duplex. Despite this, the cross-link opens and closes through reversible imine bonding. Moreover, even enzymatic incorporation of the cross-link is possible. The new principle can be used to stabilize DNA duplexes and nanostructures that otherwise require high salt concentrations, which may hinder biological applications.

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Protein

Chemical

Disease

Primary Citation of related structures
4UQG
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