ISDA: 4G5D

Deposition Date 2012-07-17

Release Date 2012-08-15

Last Version Date 2023-09-13

Entry Detail

PDB ID:

4G5D

Keywords:

OXIDOREDUCTASE

Title:

X-ray crystal structure of Prostaglandin f synthase from Leishmania major Friedlin bound to NADPH

Biological Source:

Source Organism(s):

Leishmania major (Taxon ID: 347515)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.80 Å

R-Value Free:

0.21

R-Value Work:

0.17

R-Value Observed:

0.17

Space Group:

P 1 21 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Prostaglandin f2-alpha syntha
Chain IDs:A, B
Chain Length:288
Number of Molecules:2
Biological Source:Leishmania major

UniProt ID:P22045 Pfam ID:PF00248

Ligand Molecules

NDP

Primary Citation

Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.

Moen, S.O, Fairman, J.W, Barnes, S.R, Sullivan, A, Nakazawa-Hewitt, S, Van Voorhis, W.C, Staker, B.L, Lorimer, D.D, Myler, P.J, Edwards, T.E.Show

Acta Crystallogr F Struct Biol Commun 71 609 614 (2015)

PMID: 25945716 DOI: 10.1107/S2053230X15006883

Abstact

The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.

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Primary Citation of related structures

Abstact

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