ISDA: 4FZI

Deposition Date 2012-07-06

Release Date 2012-07-18

Last Version Date 2023-09-13

Entry Detail

PDB ID:

4FZI

Keywords:

OXIDOREDUCTASE

Title:

Crystal structure of prostaglandin F synthase from Trypanosoma cruzi

Biological Source:

Source Organism(s):

Trypanosoma cruzi (Taxon ID: 353153)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.60 Å

R-Value Free:

0.22

R-Value Work:

0.18

R-Value Observed:

0.18

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Prostaglandin F synthase
Gene (Uniprot):Tc00.1047053511287.49
Chain IDs:A, B
Chain Length:290
Number of Molecules:2
Biological Source:Trypanosoma cruzi

UniProt ID:Q4DJ07 Pfam ID:PF00248 EC:1.1.1.188

Ligand Molecules

GLU

SO4

Primary Citation

Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.

Moen, S.O, Fairman, J.W, Barnes, S.R, Sullivan, A, Nakazawa-Hewitt, S, Van Voorhis, W.C, Staker, B.L, Lorimer, D.D, Myler, P.J, Edwards, T.E.Show

Acta Crystallogr F Struct Biol Commun 71 609 614 (2015)

PMID: 25945716 DOI: 10.1107/S2053230X15006883

Abstact

The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.

Legend

Protein

Chemical

Disease

Primary Citation of related structures

Abstact

Feedback Form