ISDA: 3WYE

Deposition Date 2014-08-26

Release Date 2015-08-26

Last Version Date 2024-03-20

Entry Detail

PDB ID:

3WYE

Keywords:

OXIDOREDUCTASE

Title:

Crystal Structure of chimeric engineered (2S,3S)-butanediol dehydrogenase complexed with NAD+

Biological Source:

Source Organism(s):

Klebsiella pneumoniae (Taxon ID: 573)
Corynebacterium glutamicum (Taxon ID: 1718)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.58 Å

R-Value Free:

0.19

R-Value Work:

0.18

R-Value Observed:

0.18

Space Group:

C 2 2 21

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Macromolecular Entities

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Diacetyl reductase [(S)-aceto
Gene (Uniprot):budC, budC
Mutagens:R52H, M54V,R52H, M54V,R52H, M54V,R52H, M54V,R52H, M54V,R52H, M54V,R52H, M54V
Chain IDs:A, B
Chain Length:256
Number of Molecules:2
Biological Source:Klebsiella pneumoniae, Corynebacterium glutamicum

UniProt ID:Q9ZNN8,Q48436 Pfam ID:PF00106, PF13561 EC:1.1.1.304

Ligand Molecules

NAD

Primary Citation

Crystal Structure of chimeric engineered (2S,3S)-butanediol dehydrogenase complexed with NAD+

Shimegi, T, Oyama, T, Kusunoki, M, Ui, S.Show

To be published ? ? ? (?)

Primary Citation of related structures

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