3IBP image
Deposition Date 2009-07-16
Release Date 2010-01-26
Last Version Date 2024-11-20
Entry Detail
PDB ID:
3IBP
Keywords:
CELL CYCLE
Title:
The Crystal Structure of the Dimerization Domain of Escherichia coli Structural Maintenance of Chromosomes Protein MukB
Biological Source:
Source Organism(s):
Escherichia coli (Taxon ID: 83333)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
3.10 Å
R-Value Free:
0.27
R-Value Work:
0.24
R-Value Observed:
0.24
Space Group:
P 43 2 2
3IBP validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Chromosome partition protein
Gene (Uniprot):mukB
Chain IDs:A
Chain Length:302
Number of Molecules:1
Biological Source:Escherichia coli
UniProt ID:P22523 Pfam ID:PF16330
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
MSE A MET SELENOMETHIONINE
Ligand Molecules
NH4
Primary Citation
The crystal structure of the hinge domain of the Escherichia coli structural maintenance of chromosomes protein MukB.
J. Mol. Biol. 395 11 19 (2010)
PMID: 19853611 DOI: 10.1016/j.jmb.2009.10.040

Abstact

MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosomal segregation and condensation in gamma-proteobacteria. MukB and canonical SMC proteins share a characteristic five-domain structure. Globular N- and C-terminal domains interact to form an ATP-binding cassette-like ATPase or "head" domain, which is connected to a smaller dimerization or "hinge" domain by a long, antiparallel coiled coil. In addition to mediating dimerization, this hinge region has been implicated in both conformational flexibility and dynamic protein-DNA interactions. We report here the first crystallographic model of the MukB hinge domain. This model also contains approximately 20% of the coiled-coil domain, including an unusual coiled-coil deviation. These results will facilitate studies to clarify the roles of both the hinge and the coiled-coil domains in MukB function.

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Primary Citation of related structures
3IBP
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