3B7M image
Deposition Date 2007-10-31
Release Date 2007-11-27
Last Version Date 2024-10-09
Entry Detail
PDB ID:
3B7M
Keywords:
HYDROLASE
Title:
Crystal structure of a meso-active thermo-stable cellulase (MT Cel12A) derived by making non-contiguous mutations in the active surface of the Cel12A cellulase of Rhodothermus marinus
Biological Source:
Source Organism(s):
Rhodothermus marinus (Taxon ID: 29549)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
2.10 Å
R-Value Free:
0.23
R-Value Work:
0.18
R-Value Observed:
0.18
Space Group:
P 21 21 21
3B7M validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:CELLULASE
Chain IDs:A, B, C, D
Chain Length:216
Number of Molecules:4
Biological Source:Rhodothermus marinus
UniProt ID:O33897 Pfam ID:PF01670 EC:3.2.1.4
Primary Citation
Transplantation of the active surface of a mesophile cellulase onto the structural scaffold of a homologous thermophile cellulase through engineering of a surface beta sheet
To be published ? ? ? (?)
Primary Citation of related structures
1H0B
1OA2
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