ISDA: 31MR

Deposition Date 2026-06-12

Release Date 2026-07-01

Last Version Date 2026-07-22

Entry Detail

PDB ID:

31MR

Keywords:

PROTEIN BINDING

Title:

CryoEM structure of a catalytically inactive CXC Chemokine-degrading protease SpyCEP from Streptococcus pyogenes complexed with an anti-PA-domain monoclonal antibody

Biological Source:

Source Organism(s):

Streptococcus pyogenes (Taxon ID: 1314)
Mus musculus (Taxon ID: 10090)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.07 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Cell envelope proteinase A
Chain IDs:A
Chain Length:221
Number of Molecules:1
Biological Source:Streptococcus pyogenes

Polymer Type:polypeptide(L)
Molecule:Cell envelope proteinase A
Chain IDs:B
Chain Length:1346
Number of Molecules:1
Biological Source:Streptococcus pyogenes

Polymer Type:polypeptide(L)
Molecule:Anti-PA-domain monoclonal ant
Chain IDs:C (auth: H)
Chain Length:122
Number of Molecules:1
Biological Source:Mus musculus

Polymer Type:polypeptide(L)
Molecule:Anti-PA-domain monoclonal ant
Chain IDs:D (auth: L)
Chain Length:113
Number of Molecules:1
Biological Source:Mus musculus

Ligand Molecules

Primary Citation

SpyCEP dismantles neutrophil immunity via disorder-driven chemokine remodeling and GAG targeting.

Lau, R.J, Giblin, S.P, Sugar, A, Di Maio, A, Tassini, G, Huse, K, Chorev, D, Chen, Y, Wu, G.H, Huemer, C.B, Kim, S.Y, Matthews, J, Muloud, B, Chen, L, McKenna, S, Xu, Y, Massai, L, Muzzi, C, Ferhati, X, Necchi, F, Gomes Moriel, D, Feizi, T, Liu, Y, Pease, J.E, Sriskandan, S, Matthews, S.Show

Proc.Natl.Acad.Sci.USA 123 e2520164123 e2520164123 (2026)

PMID: 42424422 DOI: 10.1073/pnas.2520164123

Primary Citation of related structures

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