ISDA: 30XM

Deposition Date 2026-05-15

Release Date 2026-08-26

Last Version Date 2026-08-26

Entry Detail

PDB ID:

30XM

Keywords:

TRANSFERASE

Title:

Engineered pyrrolysyl-tRNA synthetase (PylRS) from Methanosarcina barkeri (Mb) in complex with ATP

Biological Source:

Source Organism(s):

Methanosarcina barkeri (Taxon ID: 2208)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.55 Å

R-Value Free:

0.21

R-Value Work:

0.18

R-Value Observed:

0.18

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Pyrrolysine--tRNA ligase
Chain IDs:A, B
Chain Length:267
Number of Molecules:2
Biological Source:Methanosarcina barkeri

Ligand Molecules

ATP

Primary Citation

A Genetically Encoded Electrophilic Lysine Derivative Enables Sortase-Mediated Assembly of SUMO Activity-Based Probes

Wanka, V, Kvasha, D, Cigler, M, Heymes-Krauskopf, K, Ruckgaber, P, Heider, A, Fottner, M, Groll, M, Lang, K.Show

J.Am.Chem.Soc. ? ? ? (2026)

DOI: 10.1021/jacs.6c10081

Primary Citation of related structures

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