ISDA: 2PK8

Deposition Date 2007-04-17

Release Date 2007-05-22

Last Version Date 2024-02-21

Entry Detail

PDB ID:

2PK8

Keywords:

STRUCTURAL GENOMICS, UNKNOWN FUNCTION

Title:

Crystal structure of an uncharacterized protein PF0899 from Pyrococcus furiosus

Biological Source:

Source Organism(s):

Pyrococcus furiosus DSM 3638 (Taxon ID: 186497)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.85 Å

R-Value Free:

0.24

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

P 32 2 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Uncharacterized protein PF089
Gene (Uniprot):PF0899
Chain IDs:A
Chain Length:103
Number of Molecules:1
Biological Source:Pyrococcus furiosus DSM 3638

UniProt ID:Q8U2E0 Pfam ID:PF08967

Ligand Molecules

AUC

Primary Citation

Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.

Kelley, L.L, Dillard, B.D, Tempel, W, Chen, L, Shaw, N, Lee, D, Newton, M.G, Sugar, F.J, Jenney, F.E, Lee, H.S, Shah, C, Poole, F.L, Adams, M.W, Richardson, J.S, Richardson, D.C, Liu, Z.J, Wang, B.C, Rose, J.Show

Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63 549 552 (2007)

PMID: 17620707 DOI: 10.1107/S1744309107024049

Abstact

The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.

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Disease

Primary Citation of related structures

Abstact

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