ISDA: 2G5W

Deposition Date 2006-02-23

Release Date 2006-04-04

Last Version Date 2023-08-30

Entry Detail

PDB ID:

2G5W

Keywords:

OXIDOREDUCTASE

Title:

X-ray crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 (AtOPR3) in complex with 8-iso prostaglandin A1 and its cofactor, flavin mononucleotide.

Biological Source:

Source Organism(s):

Arabidopsis thaliana (Taxon ID: 3702)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.58 Å

R-Value Free:

0.26

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:12-oxophytodienoate reductase
Gene (Uniprot):OPR3
Chain IDs:A, B
Chain Length:391
Number of Molecules:2
Biological Source:Arabidopsis thaliana

UniProt ID:Q9FUP0 Pfam ID:PF00724 EC:1.3.1.42

Ligand Molecules

8PG

FMN

Primary Citation

Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A(1).

Han, B.W, Malone, T.E, Kim, D.J, Bingman, C.A, Kim, H.J, Fox, B.G, Phillips, G.N.Show

Proteins 79 3236 3241 (2011)

PMID: 21915915 DOI: 10.1002/prot.23153

Abstact

12-Oxophytodienoate reductase 3 (OPR3), one of the enzymes involved in the biosynthesis of the plant hormone jasmonic acid (JA), catalyzes the reduction of the cyclopentenone ring of (9S,13S)-12-oxophytodienoate [(9S,13S)-OPDA]. However, there has been no structural information about the interaction between OPRs and the physiologically relevant (9S,13S)-OPDA. Here we report the crystal structure of Arabidopsis thaliana OPR3 in complex with 8-iso prostaglandin A1 (8-iso PGA1) which has the same stereochemistry in the cyclopentenone ring as in the physiologically relevant 9S,13S-OPDA. This structure reveals a new binding mode for substrate that likely contributes to the relaxed stereospecificity observed for AtOPR3.

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Primary Citation of related structures

Abstact

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