28FS image
Deposition Date 2026-06-24
Release Date 2026-10-14
Last Version Date 2026-10-14
Entry Detail
PDB ID:
28FS
Keywords:
OXIDOREDUCTASE
Title:
Cryo-EM structure of hydrogenase-2 in complex with L1 (left)
Biological Source:
Source Organism(s):
synthetic construct (Taxon ID: 32630)
Escherichia coli K-12 (Taxon ID: 83333)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.49 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
28FS validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Hydrogenase-2 small chain
Gene (Uniprot):hybO
Chain IDs:B (auth: A), D (auth: C)
Chain Length:298
Number of Molecules:2
Biological Source:Escherichia coli K-12
UniProt ID:P69741 EC:1.12.99.6
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:Hydrogenase-2 large chain
Gene (Uniprot):hybC
Chain IDs:C (auth: B), E (auth: D)
Chain Length:567
Number of Molecules:2
Biological Source:Escherichia coli K-12
UniProt ID:P0ACE0 EC:1.12.99.6
Polymer Type:polypeptide(L)
Molecule:binder L1
Chain IDs:A (auth: E)
Chain Length:82
Number of Molecules:1
Biological Source:synthetic construct
Ligand Molecules
F3S
FCO
NI
SF4
Primary Citation
De Novo Design of a Protein Binder to Probe Gas Channel and Enhance the Oxygen Tolerance of [NiFe]-Hydrogenase.
Angew.Chem.Int.Ed.Engl. ? e6395554 e6395554 (2026)
PMID: 42655993 DOI: 10.1002/anie.6395554
Primary Citation of related structures
28FS
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