ISDA: 25XO

Deposition Date 2026-04-22

Release Date 2026-08-05

Last Version Date 2026-08-05

Entry Detail

PDB ID:

25XO

Keywords:

VIRAL PROTEIN/RNA

Title:

Cryo-EM structure of the Crimean-Congo hemorrhagic fever virus full-length L protein RdRP elongation complex (EC14b form)

Biological Source:

Source Organism(s):

Orthonairovirus haemorrhagiae (Taxon ID: 3052518)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Spodoptera frugiperda

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.18 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA-directed RNA polymerase L
Chain IDs:A
Chain Length:3989
Number of Molecules:1
Biological Source:Orthonairovirus haemorrhagiae

UniProt ID:A0A068JD44 EC:2.7.7.48,3.4.19.12

Polymer Type:polyribonucleotide
Molecule:RNA (38-MER)
Chain IDs:B, F
Chain Length:38
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (5'-R(*GP*GP*AP*UP*AP*UP*
Chain IDs:C
Chain Length:14
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (5'-R(*UP*CP*UP*CP*AP*AP*
Chain IDs:D
Chain Length:18
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (5'-R(*GP*GP*GP*A)-3')
Chain IDs:E
Chain Length:4
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

RNA synthesis and substrate analog inhibition in the CCHFV polymerase

Jia, H, Tang, B, Liu, S, Wen, X, Wu, F, Hu, X, Zhou, H, Chong, T, Lv, L, Liu, Q, Rao, G, Wei, M, Jing, X, Cao, S, Deng, F, Hu, Z, Shu, B, Gong, R, Wu, J, Wang, M, Gong, P.Show

Nature ? ? ? (2026)

DOI: 10.1038/s41586-026-10913-w

Primary Citation of related structures

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