ISDA: 25QL

Deposition Date 2026-04-14

Release Date 2026-08-26

Last Version Date 2026-08-26

Entry Detail

PDB ID:

25QL

Keywords:

ANTIVAL PROTEIN/RNA/DNA

Title:

Cryo-EM structure of TasH-tigRNA-MM5 dsDNA complex

Biological Source:

Source Organism(s):

Salicola phage CGphi29 (Taxon ID: 754067)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.02 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Putative nuclease
Chain IDs:A, B
Chain Length:354
Number of Molecules:2
Biological Source:Salicola phage CGphi29

Polymer Type:polyribonucleotide
Molecule:RNA (37-MER)
Chain IDs:C
Chain Length:37
Number of Molecules:1
Biological Source:Salicola phage CGphi29

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (38-MER)
Chain IDs:D (auth: E)
Chain Length:38
Number of Molecules:1
Biological Source:Salicola phage CGphi29

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (38-MER)
Chain IDs:E (auth: F)
Chain Length:38
Number of Molecules:1
Biological Source:Salicola phage CGphi29

Ligand Molecules

Primary Citation

Molecular basis of single-mismatch-induced nuclease-to-nickase conversion in TIGR-TasH.

Zhou, R, Zhan, Y, Sun, Y, Wang, R, Wang, T, Liu, Z, Shan, Z, Li, X, Zhang, S, Sun, N, Zhang, H, Yuan, Z, Yang, J.Show

Nucleic Acids Res. 54 ? ? (2026)

PMID: 42531075 DOI: 10.1093/nar/gkag743

Primary Citation of related structures

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