ISDA: 23VW

Deposition Date 2026-02-21

Release Date 2026-09-02

Last Version Date 2026-09-02

Entry Detail

PDB ID:

23VW

Keywords:

DNA BINDING PROTEIN

Title:

Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex

Biological Source:

Source Organism(s):

Thermanaerosceptrum fracticalcis (Taxon ID: 1712410)
DNA molecule (Taxon ID: 2853804)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.39 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated DNA-binding
Chain IDs:A
Chain Length:577
Number of Molecules:1
Biological Source:Thermanaerosceptrum fracticalcis

Polymer Type:polyribonucleotide
Molecule:RNA (59-MER)
Chain IDs:D (auth: B)
Chain Length:59
Number of Molecules:1
Biological Source:Thermanaerosceptrum fracticalcis

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:C
Chain Length:39
Number of Molecules:1
Biological Source:DNA molecule

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (35-MER)
Chain IDs:B (auth: D)
Chain Length:39
Number of Molecules:1
Biological Source:DNA molecule

Ligand Molecules

Primary Citation

Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation

Morinaga, H, Omura, S.N, Alfonse, L, Ornstein, A, Kobayashi, T.A, Onishi, K, Makarova, K.S, Shmakov, S.A, Munoz, G, Garrity, A.J, DiTommaso, T, Koonin, E.V, Maben, Z, Nureki, O.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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