23VV image
Deposition Date 2026-02-21
Release Date 2026-09-02
Last Version Date 2026-09-02
Entry Detail
PDB ID:
23VV
Keywords:
DNA BINDING PROTEIN
Title:
Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the NTS-cleaving state
Biological Source:
Source Organism(s):
bacterium (Taxon ID: 1869227)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.84 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
23VV validation report missing
Macromolecular Entities
Polymer Type:polypeptide(L)
Molecule:UnCas12m4a
Chain IDs:C (auth: A)
Chain Length:614
Number of Molecules:1
Biological Source:bacterium
Polymer Type:polyribonucleotide
Molecule:RNA (56-MER)
Chain IDs:D (auth: B)
Chain Length:56
Number of Molecules:1
Biological Source:bacterium
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:B (auth: C)
Chain Length:39
Number of Molecules:1
Biological Source:bacterium
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:A (auth: D)
Chain Length:39
Number of Molecules:1
Biological Source:bacterium
Ligand Molecules
MG
PST
ZN
Primary Citation
Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation
To Be Published ? ? ? (?)
Primary Citation of related structures
23VV
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