ISDA: 23VU

Deposition Date 2026-02-21

Release Date 2026-09-02

Last Version Date 2026-09-02

Entry Detail

PDB ID:

23VU

Keywords:

DNA BINDING PROTEIN

Title:

Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the TS-cleaving state

Biological Source:

Source Organism(s):

bacterium (Taxon ID: 1869227)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.69 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:UnCas12m4a
Chain IDs:A
Chain Length:614
Number of Molecules:1
Biological Source:bacterium

Polymer Type:polyribonucleotide
Molecule:RNA (56-MER)
Chain IDs:B
Chain Length:56
Number of Molecules:1
Biological Source:bacterium

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:C
Chain Length:39
Number of Molecules:1
Biological Source:bacterium

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (39-MER)
Chain IDs:D
Chain Length:39
Number of Molecules:1
Biological Source:bacterium

Ligand Molecules

Primary Citation

Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation

Morinaga, H, Omura, S.N, Alfonse, L, Ornstein, A, Kobayashi, T.A, Onishi, K, Makarova, K.S, Shmakov, S.A, Munoz, G, Garrity, A.J, DiTommaso, T, Koonin, E.V, Maben, Z, Nureki, O.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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