22FN image
Deposition Date 2026-01-08
Release Date 2026-04-29
Last Version Date 2026-04-29
Entry Detail
PDB ID:
22FN
Keywords:
DNA BINDING PROTEIN
Title:
Cryo-EM structure of AsCas12a in complex with crDNA and RNA target
Biological Source:
Source Organism(s):
Acidaminococcus sp. BV3L6 (Taxon ID: 1111120)
synthetic construct (Taxon ID: 32630)
Expression System(s):
Escherichia coli 'bl21-gold(de3)plyss ag'
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.17 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
22FN validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):cas12a
Mutagens:M537R/F870L
Chain IDs:B (auth: A)
Chain Length:1307
Number of Molecules:1
Biological Source:Acidaminococcus sp. BV3L6
UniProt ID:U2UMQ6
Polymer Type:polyribonucleotide
Molecule:RNA (5'-R(P*GP*AP*CP*AP*GP*CP
Chain IDs:A (auth: B)
Chain Length:45
Number of Molecules:1
Biological Source:synthetic construct
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (41-MER)
Chain IDs:C
Chain Length:43
Number of Molecules:1
Biological Source:synthetic construct
Ligand Molecules
NA
Primary Citation
Cryo-EM structure of AsCas12a in complex with crDNA and RNA target
To Be Published ? ? ? (?)
Primary Citation of related structures
22FN
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