ISDA: 21EA

Deposition Date 2025-12-09

Release Date 2026-07-01

Last Version Date 2026-07-01

Entry Detail

PDB ID:

21EA

Keywords:

RNA BINDING PROTEIN/RNA/DNA

Title:

Cryo-EM structure of AtCas9-sgRNA-underwound DNA (TATA PAM) ternary complex

Biological Source:

Source Organism(s):

Alicyclobacillus tolerans (Taxon ID: 90970)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.65 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Mutagens:D8A, H617A, N640A
Chain IDs:A
Chain Length:1154
Number of Molecules:1
Biological Source:Alicyclobacillus tolerans

Polymer Type:polyribonucleotide
Molecule:sgRNA
Chain IDs:B
Chain Length:117
Number of Molecules:1
Biological Source:Alicyclobacillus tolerans

Polymer Type:polydeoxyribonucleotide
Molecule:TS DNA
Chain IDs:C
Chain Length:35
Number of Molecules:1
Biological Source:Alicyclobacillus tolerans

Polymer Type:polydeoxyribonucleotide
Molecule:NTS DNA
Chain IDs:D
Chain Length:35
Number of Molecules:1
Biological Source:Alicyclobacillus tolerans

Ligand Molecules

Primary Citation

Structural basis of AtCas9 recognition of PAM mutants in underwound DNA topology

Duan, M, Meng, B, Zhou, L, Wu, L.J, Tong, X, Huang, D, Yin, H, Liu, Z.J, Zhang, Y.Show

Nat.Struct.Mol.Biol. ? ? ? (2026)

DOI: 10.1038/s41594-026-01831-6

Primary Citation of related structures

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