ISDA: 1DIW

Deposition Date 1999-11-30

Release Date 2000-03-24

Last Version Date 2024-02-07

Entry Detail

PDB ID:

1DIW

Keywords:

TOXIN

Title:

THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH GALACTOSE

Biological Source:

Source Organism(s):

Clostridium tetani (Taxon ID: 1513)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.00 Å

R-Value Free:

0.26

R-Value Work:

0.21

R-Value Observed:

0.21

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:TETANUS TOXIN HC
Gene (Uniprot):tetX
Chain IDs:A
Chain Length:441
Number of Molecules:1
Biological Source:Clostridium tetani

UniProt ID:P04958 Pfam ID:PF07953, PF07951 EC:3.4.24.68

Ligand Molecules

GAL

Primary Citation

The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding.

Emsley, P, Fotinou, C, Black, I, Fairweather, N.F, Charles, I.G, Watts, C, Hewitt, E, Isaacs, N.W.Show

J. Biol. Chem. 275 8889 8894 (2000)

PMID: 10722735 DOI: 10.1074/jbc.275.12.8889

Abstact

The entry of tetanus neurotoxin into neuronal cells proceeds through the initial binding of the toxin to gangliosides on the cell surface. The carboxyl-terminal fragment of the heavy chain of tetanus neurotoxin contains the ganglioside-binding site, which has not yet been fully characterized. The crystal structures of native H(C) and of H(C) soaked with carbohydrates reveal a number of binding sites and provide insight into the possible mode of ganglioside binding.

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Primary Citation of related structures

Abstact

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