1RFE image
Deposition Date 2003-11-08
Release Date 2004-12-28
Last Version Date 2025-03-26
Entry Detail
PDB ID:
1RFE
Keywords:
STRUCTURAL GENOMICS, UNKNOWN FUNCTION
Title:
Crystal structure of conserved hypothetical protein Rv2991 from Mycobacterium tuberculosis
Biological Source:
Source Organism(s):
Mycobacterium tuberculosis (Taxon ID: 83332)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
2.00 Å
R-Value Free:
0.28
R-Value Work:
0.20
R-Value Observed:
0.20
Space Group:
P 43 2 2
1RFE validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:hypothetical protein Rv2991
Gene (Uniprot):Rv2991
Chain IDs:A
Chain Length:162
Number of Molecules:1
Biological Source:Mycobacterium tuberculosis
UniProt ID:O53240 Pfam ID:PF01243
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
CSX A CYS S-OXY CYSTEINE
MSE A MET SELENOMETHIONINE
Primary Citation
The crystal structure of Rv2991 from Mycobacterium tuberculosis: An F420binding protein with unknown function.
J. Struct. Biol. ? ? ? (2019)
PMID: 30890426 DOI: 10.1016/j.jsb.2019.03.006

Abstact

The crystal structure of the conserved hypothetical protein Rv2991 from Mycobacterium tuberculosis has been solved by SAD using seleno-methionine substituted protein. The dimeric biological assembly and the sequence and fold conservation are typical of F420 cofactor binding enzymes. Despite Rv2991 still being of unknown function, sequence and structural comparison with similar proteins enable a role to be proposed for its C-terminal stretch of residues in recognizing and orienting the substrate. In addition, the C-terminus is involved in both protein folding and determining the size of the active site cavity.

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Primary Citation of related structures
1RFE
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