ISDA: 1QNE

Deposition Date 1999-10-14

Release Date 2000-02-07

Last Version Date 2026-07-15

Entry Detail

PDB ID:

1QNE

Keywords:

TATA BOX-BINDING PROTEIN (TBP)

Title:

Crystal structure of the Adenovirus major late promoter TATA box bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2).

Biological Source:

Source Organism(s):

ARABIDOPSIS THALIANA (Taxon ID: 3702)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.90 Å

R-Value Free:

0.26

R-Value Work:

0.19

R-Value Observed:

0.19

Space Group:

P 1 21 1

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:TRANSCRIPTION INITIATION FACT
Gene (Uniprot):TBP1
Chain IDs:A, B
Chain Length:200
Number of Molecules:2
Biological Source:ARABIDOPSIS THALIANA

UniProt ID:P28147 Pfam ID:PF00352

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*GP*CP*TP*AP*TP*AP*
Chain IDs:C, E
Chain Length:14
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*TP*GP*CP*CP*CP*TP*
Chain IDs:D, F
Chain Length:14
Number of Molecules:2
Biological Source:synthetic construct

Primary Citation

Co-crystal structure of TBP recognizing the minor groove of a TATA element.

Patikoglou, G.A, Kim, J.L, Sun, L, Yang, S.-H, Kodadek, T, Burley, S.K.Show

Nature 365 520 527 (1993)

PMID: 8413605 DOI: 10.1038/365520a0

Abstact

The three-dimensional structure of a TATA-box binding polypeptide complexed with the TATA element of the adenovirus major late promoter has been determined by X-ray crystallography at 2.25 A resolution. Binding of the saddle-shaped protein induces a conformational change in the DNA, inducing sharp kinks at either end of the sequence TATAAAAG. Between the kinks, the right-handed double helix is smoothly curved and partially unwound, presenting a widened minor groove to TBP's concave, antiparallel beta-sheet. Side-chain/base interactions are restricted to the minor groove, and include hydrogen bonds, van der Waals contacts and phenylalanine-base stacking interactions.

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Primary Citation of related structures

Abstact

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