1IXH image
Deposition Date 1996-08-01
Release Date 1998-02-04
Last Version Date 2024-05-22
Entry Detail
PDB ID:
1IXH
Keywords:
PHOSPHATE TRANSPORT
Title:
PHOSPHATE-BINDING PROTEIN (PBP) COMPLEXED WITH PHOSPHATE
Biological Source:
Source Organism(s):
Escherichia coli (Taxon ID: 562)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
0.98 Å
R-Value Free:
0.14
R-Value Observed:
0.11
Space Group:
P 21 21 21
1IXH validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Structural Superimposition Protein Blast
Polymer Type:polypeptide(L)
Molecule:PHOSPHATE-BINDING PROTEIN
Chain IDs:A
Chain Length:321
Number of Molecules:1
Biological Source:Escherichia coli
UniProt ID:P0AG82
Ligand Molecules
PO4
Primary Citation
A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes.
Nat. Struct. Biol. 4 519 522 (1997)
PMID: 9228942 DOI: 10.1038/nsb0797-519

Abstact

A very short hydrogen bond between an Asp and a phosphate is established in two high resolution structures (0.98 and 1.05 A). A mutant complex that changes the Asp to an Asn, which forms a normal hydrogen bond, has a similar free energy of binding to the wild type complex, suggesting that the contribution of the short hydrogen bond is not extraordinarily strong.

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Disease

Primary Citation of related structures
1IXH
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