ISDA: 1HJK

Deposition Date 1997-05-30

Release Date 1997-10-15

Last Version Date 2024-11-13

Entry Detail

PDB ID:

1HJK

Keywords:

HYDROLASE

Title:

ALKALINE PHOSPHATASE MUTANT H331Q

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.30 Å

R-Value Free:

0.2

R-Value Work:

0.17

R-Value Observed:

0.17

Space Group:

I 2 2 2

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:ALKALINE PHOSPHATASE
Gene (Uniprot):phoA
Mutagens:H331Q
Chain IDs:A, B
Chain Length:449
Number of Molecules:2
Biological Source:Escherichia coli

UniProt ID:P00634 Pfam ID:PF00245 EC:3.1.3.1

Modified Residue

Compound ID	Chain ID	Parent Comp ID	Details	2D Image
SEP	A	SER	PHOSPHOSERINE

Ligand Molecules

SO4

Primary Citation

Trapping and visualization of a covalent enzyme-phosphate intermediate.

Murphy, J.E, Stec, B, Ma, L, Kantrowitz, E.R.Show

Nat. Struct. Biol. 4 618 622 (1997)

PMID: 9253408 DOI: 10.1038/nsb0897-618

Abstact

Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

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Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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