ISDA: 1GMG

Deposition Date 2001-09-13

Release Date 2002-09-12

Last Version Date 2024-10-09

Entry Detail

PDB ID:

1GMG

Keywords:

TRANSCRIPTION REGULATION

Title:

ALANINE 31 PROLINE MUTANT OF ROP PROTEIN, MONOCLINIC FORM

Biological Source:

Source Organism(s):

ESCHERICHIA COLI (Taxon ID: 562)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.90 Å

R-Value Free:

0.23

R-Value Work:

0.18

R-Value Observed:

0.18

Space Group:

C 1 2 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:REGULATORY PROTEIN ROP
Gene (Uniprot):rop
Mutagens:YES
Chain IDs:A, B
Chain Length:63
Number of Molecules:2
Biological Source:ESCHERICHIA COLI

UniProt ID:P03051 Pfam ID:PF01815

Primary Citation

Structure Determination of a Small Protein Through a 23-Dimensional Molecular-Replacement Search.

Glykos, N.M, Kokkinidis, M.Show

Acta Crystallogr. D Biol. Crystallogr. 59 709 ? (2003)

PMID: 12657790 DOI: 10.1107/S0907444903002889

Abstact

The crystal structure of a 4-alpha-helical bundle protein has been determined by the application of a 23-dimensional molecular-replacement search performed using a stochastic method. The search model for the calculation was a 26-residue-long polyalanine helix amounting to less than 13% of the total number of atoms in the asymmetric unit of the target crystal structure. The crystal structure determination procedure is presented in detail, with emphasis on the molecular-replacement calculations.

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Primary Citation of related structures

Abstact

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