ISDA: 12FK

Deposition Date 2026-04-02

Release Date 2026-04-15

Last Version Date 2026-04-15

Entry Detail

PDB ID:

12FK

Keywords:

DNA BINDING PROTEIN

Title:

Ancestral Reconstruction of a Homing Endonuclease

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli, Synthetic construct

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.17 Å

R-Value Free:

0.28

R-Value Work:

0.23

R-Value Observed:

0.23

Space Group:

P 1 21 1

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:aHE31
Chain IDs:A
Chain Length:306
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*GP*AP*AP*CP*CP*TP
Chain IDs:B
Chain Length:24
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*TP*GP*AP*AP*GP*AP
Chain IDs:C
Chain Length:24
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Ancestral Homing Endonuclease reconstructions

Stoddard, B.Show

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Primary Citation of related structures

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