ISDA: 10ZP

Deposition Date 2026-02-13

Release Date 2026-10-07

Last Version Date 2026-10-07

Entry Detail

PDB ID:

10ZP

Keywords:

RNA BINDING PROTEIN

Title:

Cryo-EM structure of TccCas13a-crRNA-target RNA ternary complex

Biological Source:

Source Organism(s):

Thermoclostridium caenicola (Taxon ID: 659425)

Expression System(s):

Escherichia coli 'bl21-gold(de3)plyss ag'

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.13 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endoribonuc
Gene (Uniprot):SAMN05444373_102315
Mutagens:L668K, E830H, E877R, V1015K, R1111A, H1116A
Chain IDs:A
Chain Length:1225
Number of Molecules:1
Biological Source:Thermoclostridium caenicola

UniProt ID:A0A1M6GDI0

Polymer Type:polyribonucleotide
Molecule:RNA (95-MER)
Chain IDs:B
Chain Length:95
Number of Molecules:1
Biological Source:Thermoclostridium caenicola

Ligand Molecules

Primary Citation

Structural basis and rational engineering of a thermophilic Cas13a for robust RNA detection.

Oladeji, S.J, Gonzalez, D.H, Deng, X, You, Z, Zhang, Y, Golla, D.A, Smith, J, Zhang, Y, Gao, X, Lillehoj, P.B, Gao, Y.Show

Cell Chem Biol 34 1 14 (2026)

DOI: 10.1016/j.chembiol.2026.09.012

Primary Citation of related structures

Feedback Form