10ZB image
Deposition Date 2026-02-12
Release Date 2026-05-20
Last Version Date 2026-05-20
Entry Detail
PDB ID:
10ZB
Keywords:
TRANSFERASE
Title:
Room-temperature X-ray structure of E53Q mutant of Thermus thermophilus serine hydroxymethyltransferase (TthSHMT) in complex with PLP-L-Ser external aldimine and tetrahydrofolate (THF)
Biological Source:
Source Organism(s):
Thermus thermophilus (Taxon ID: 274)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
1.80 Å
R-Value Free:
0.18
R-Value Work:
0.15
R-Value Observed:
0.15
Space Group:
P 1 21 1
10ZB validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Serine hydroxymethyltransfera
Gene (Uniprot):glyA
Mutagens:E53Q
Chain IDs:A
Chain Length:405
Number of Molecules:1
Biological Source:Thermus thermophilus
UniProt ID:A0AAD1KUU5 EC:2.1.2.1
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Serine hydroxymethyltransfera
Gene (Uniprot):glyA
Chain IDs:B
Chain Length:405
Number of Molecules:1
Biological Source:Thermus thermophilus
UniProt ID:A0AAD1KUU5 EC:2.1.2.1
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
LLP A LYS modified residue
Ligand Molecules
ACT
KOU
SO4
THG
Primary Citation
Mutation of active site glutamate in serine hydroxymethyltransferase allows trapping a reactive intermediate: a combined neutron and X-ray crystallography study
Rsc Adv 16 22600 22609 (2026)
DOI: 10.1039/D6RA01814A
Primary Citation of related structures
10ZB
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