ELECTRON MICROSCOPY

Sample

Ternary complex of dimeric NPR1 with salicylic acid and MED15A KIX domain

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Sample was manually applied to grid and blotted outside of the Vitrobot twice before being applied once and two-side blotted with two layers of blotting papers with blot force 0 for 9 seconds within the Vitrobot chamber. Grids used: Amorphous Ni-Titanium Holey Foil Grids from Molecular Dimensions; Gold support grid, Ni-Ti holey foil, 300 mesh, 1.2/1.3 Aperture Pitch
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 773558
Reported Resolution (Å) 3.2
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 60
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC ?
RECONSTRUCTION cryoSPARC ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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