X-RAY DIFFRACTION

Crystallization Details
Method pH Temprature Details
X-RAY DIFFRACTION ? 293 Drops contained 400 nl of 1 mM RNA and 400 nl of buffer containing 10% 2-methyl-2,4-pentanediol (MPD), 12 mM spermine 4 HCl, 80 mM potassium chloride, 12 mM NaCl and 40 mM sodium cacodylate, pH 7.0.
Unit Cell:
a: 30.787 Å b: 90.821 Å c: 48.947 Å α: 90.000° β: 98.723° γ: 90.000°
Symmetry:
Space Group: P 1 21 1
Crystal Properties:
Matthew's Coefficient: 2.19 Solvent Content: 43.81
Refinement Statistics
Diffraction ID Structure Solution Method Cross Validation Method Resolution Limit (High) Resolution Limit (Low) Number of Reflections (Observed) Number of Reflections (R-free) Percent Reflections (Observed) R-Work R-Free Mean Isotropic
X-RAY DIFFRACTION SAD FREE R-VALUE 1.54 30.43 36163 3532 91.79 0.2455 0.2835 29.42
Data Collection
Overall
Resolution Limit (High) Resolution Limit (Low) Percent Possible (Observed) R Merge I (Observed) R Sym I (Observed) Net I Over Average Sigma (I) Redundancy Number Reflections (All) Number Reflections (Observed) Observed Criterion Sigma (F) Observed Criterion Sigma (I) B (Isotropic) From Wilson Plot
1.54 30.43 91.80 ? ? 10.99 13.2 ? 36267 ? ? 22.78
Highest Resolution Shell
# Resolution Limits (Low) Resolution Limits (High) Percent Possible (All) Percent Possible (Observed) R-Sym I (Observed) Mean I Over Sigma (Observed) Redundancy Number Unique Reflections (All)
1 1.54 1.56 ? ? ? ? ? ?
Diffraction
Diffraction experiment
Crystal ID Scattering Type Data Collection Temprature Detector Detector Type Details Collection Date Monochromator Protocol
1 100 K ?
Radiation Source
Source Type Wavelength List Synchrotron Site Beamline
SYNCHROTRON APS BEAMLINE 21-ID-D 0.9187 APS 21-ID-D
Software
Software Name Purpose Version
PHENIX refinement 1.21.1_5286
DIALS data reduction .
DIALS data scaling .
HKL2Map phasing .
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