ISDA: 9YDI

X-RAY DIFFRACTION

Crystallization Details

Method	pH	Temprature	Details
X-RAY DIFFRACTION	?	293	grown in drops containing 0.3 microliter of 1.0 mM RNA and 0.3 microliter of 10% 2-methyl-2,4-pentanediol (MPD), 12 mM spermine-4HCl, 20 mM MgCl2, 80 mM KCl, 40 mM sodium cacodylate, pH 7.0. The reservoirs contained 70 microliters 40% 2-methyl-2,4-pentanediol

Unit Cell:

a: 41.168 Å b: 34.964 Å c: 31.910 Å α: 90.000° β: 128.929° γ: 90.000°

Symmetry:

Space Group: C 1 2 1

Crystal Properties:

Matthew's Coefficient: 2.32 Solvent Content: 46.97

Refinement Statistics

Diffraction ID	Structure Solution Method	Cross Validation Method	Resolution Limit (High)	Resolution Limit (Low)	Number of Reflections (Observed)	Number of Reflections (R-free)	Percent Reflections (Observed)	R-Work	R-Free	Mean Isotropic
X-RAY DIFFRACTION	MOLECULAR REPLACEMENT	FREE R-VALUE	1.03	16.01	33859	1714	98.86	0.1724	0.1831	15.39

Data Collection

Overall

Resolution Limit (High)	Resolution Limit (Low)	Percent Possible (Observed)	R Merge I (Observed)	R Sym I (Observed)	Net I Over Average Sigma (I)	Redundancy	Number Reflections (All)	Number Reflections (Observed)	Observed Criterion Sigma (F)	Observed Criterion Sigma (I)	B (Isotropic) From Wilson Plot
1.03	16.01	98.9	?	?	4.41	4.5	?	37549	?	?	10.01

Highest Resolution Shell

#	Resolution Limits (Low)	Resolution Limits (High)	Percent Possible (All)	Percent Possible (Observed)	R-Sym I (Observed)	Mean I Over Sigma (Observed)	Redundancy	Number Unique Reflections (All)
1	1.03	1.06	?	?	?	?	?	?

Diffraction

Diffraction experiment

Crystal ID	Scattering Type	Data Collection Temprature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1		100 K			?

Radiation Source

Source	Type	Wavelength List	Synchrotron Site	Beamline
LIQUID ANODE	BRUKER METALJET	1.3412	?	?

Software