ELECTRON MICROSCOPY

Sample

Cryo-EM Structure of the 14-Mer ATPase Complex YsaN from the Type III Secretion System of Yersinia enterocolitica

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 123350
Reported Resolution (Å) 4.40
Resolution Method OTHER
Other Details The Composite map was generated using a consensus map and a focus map in ChimeraX using volume maximum command. The resolution was estimated using map alone (d99). The software used for the resolution estimation is Mtriage (Phenix).
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS TALOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 150000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.6.2
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 4.6.2
MODEL FITTING UCSF ChimeraX 1.9
MODEL FITTING Coot 0.9.8.95
INITIAL EULER ASSIGNMENT cryoSPARC 4.6.2
FINAL EULER ASSIGNMENT cryoSPARC 4.6.2
CLASSIFICATION cryoSPARC 4.6.2
RECONSTRUCTION cryoSPARC 4.6.2
RECONSTRUCTION UCSF ChimeraX ?
MODEL REFINEMENT PHENIX 1.21.2_5419:
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
Feedback Form
Name
Email
Institute
Feedback