ELECTRON MICROSCOPY

Sample

SARM1

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 2.5ul
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 110926
Reported Resolution (Å) 3.3
Resolution Method FSC 0.143 CUT-OFF
Other Details Non-uniform refinement
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space RECIPROCAL
Refinement Protocol RIGID BODY FIT
Refinement Target Cross-correlation
Overall B Value 62.3
Fitting Procedure ?
Details Rigid body fitting of TIR monomers was done in Chimera, followed by realspace refinement in Coot, and finally refinement in Phenix.
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 43
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 120000
Calibrated Magnification 213000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.7.1
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 4.7.1
MODEL FITTING Coot 0.9.8.92
INITIAL EULER ASSIGNMENT cryoSPARC 4.7.1
FINAL EULER ASSIGNMENT cryoSPARC 4.7.1
CLASSIFICATION cryoSPARC 4.7.1
RECONSTRUCTION cryoSPARC ?
MODEL REFINEMENT PHENIX 1.21.1_5286:
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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