ELECTRON MICROSCOPY

Sample

DDK-salt stripped phosphorylated MCM2-7 double hexamer bound DNA

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Wait time: 30 s Blotting time: 1.5 s Blot force: +2
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 64506
Reported Resolution (Å) 3.30
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON III (4k x 4k)
Electron Dose (electrons/Å2) 58.4
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 81000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz ?
MODEL REFINEMENT PHENIX 1.21.2_5419
IMAGE ACQUISITION EPU ?
CTF CORRECTION CTFFIND 4.1.14
INITIAL EULER ASSIGNMENT RELION 4.0
FINAL EULER ASSIGNMENT RELION 4.0
CLASSIFICATION RELION 4.0
RECONSTRUCTION RELION 4.0
RECONSTRUCTION PHENIX 1.21.2-5419
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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