ELECTRON MICROSCOPY

Sample

VSIV L:P:N complex

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 34000
Reported Resolution (Å) 3.8
Resolution Method FSC 0.143 CUT-OFF
Other Details 3D Flexible refinement
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target Cross-correlation coefficient
Overall B Value ?
Fitting Procedure ?
Details L-P from 6U1X was rigid-body fitted in the map. N0-P from 3PMK (chains D and O) was rigid-body fitted in the map after deletion of the C-loop (aa 342-374). The model was then locally edited and refined to introduce mutations and solve clashes.
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 55.99
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2400
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.6
IMAGE ACQUISITION EPU 3.5.1
CTF CORRECTION cryoSPARC 4.6
MODEL FITTING UCSF ChimeraX 1.9.dev202410260116
INITIAL EULER ASSIGNMENT cryoSPARC 4.6
FINAL EULER ASSIGNMENT cryoSPARC 4.6
RECONSTRUCTION cryoSPARC 4.6
MODEL REFINEMENT Coot 0.9.8.93
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION CTF estimation and correction with cryoSPARC
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