ELECTRON MICROSCOPY

Sample

Homotrimer of the essential Mycoplasma pneumoniae lipoprotein Mpn444

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Nominal blot force -3 Wait time 10 s Blotting time 14 s. Before freezing, Whatman 595 filter papers were incubated for 1 h in the Vitrobot chamber at 100% relative humidity and 278K.
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 506291
Reported Resolution (Å) 5.85
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 3600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.5.3
IMAGE ACQUISITION SerialEM v4.1.0 beta
CTF CORRECTION cryoSPARC v4.5.3
MODEL FITTING UCSF ChimeraX ?
INITIAL EULER ASSIGNMENT cryoSPARC v4.5.3
FINAL EULER ASSIGNMENT cryoSPARC v4.5.3
CLASSIFICATION cryoSPARC v4.5.3
RECONSTRUCTION cryoSPARC v4.5.3
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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