ELECTRON MICROSCOPY

Sample

Essential Mycoplasma pneumoniae lipoprotein Mpn436

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Nominal blot force -3 Wait time 10 s Blotting time 8 to 14 s. Before freezing, Whatman 595 filter papers were incubated for 1 h in the Vitrobot chamber at 100% relative humidity and 278K.
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 468211
Reported Resolution (Å) 3.65
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol AB INITIO MODEL
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 3600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.5.3
IMAGE ACQUISITION SerialEM v4.1.0 beta
CTF CORRECTION cryoSPARC v4.5.3
MODEL FITTING ISOLDE ?
INITIAL EULER ASSIGNMENT cryoSPARC v4.5.3
FINAL EULER ASSIGNMENT cryoSPARC v4.5.3
CLASSIFICATION cryoSPARC v4.5.3
RECONSTRUCTION cryoSPARC v4.5.3
MODEL REFINEMENT PHENIX ?
MODEL REFINEMENT Coot ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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