ELECTRON MICROSCOPY

Sample

alpha4-beta3-delta GABA-A receptor in complex with DS2-Me, GABA, nanobody Nb24 and megabody Mb30

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 110542
Reported Resolution (Å) 3.04
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 53.6
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2200
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION ARP/wARP 1.0.9
CTF CORRECTION ARP/wARP 1.0.9
MODEL FITTING UCSF ChimeraX 1.8
MODEL REFINEMENT PHENIX 1.20.1_4487
INITIAL EULER ASSIGNMENT cryoSPARC 4.4.1 to 4.7.1
FINAL EULER ASSIGNMENT cryoSPARC 4.4.1 to 4.7.1
CLASSIFICATION cryoSPARC 4.4.1 to 4.7.1
RECONSTRUCTION cryoSPARC 4.4.1 to 4.7.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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