ELECTRON MICROSCOPY

Sample

HnCsoSCA

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 4C, 100% relative humidity, delay time 0s, blot time 3s, blot force 0
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 486613
Reported Resolution (Å) 2.13
Resolution Method FSC 0.143 CUT-OFF
Other Details GS-FSC resolutution calculated with the auto-tightened mask in CryoSPARC.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space RECIPROCAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 64.757
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 1800
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 130000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz 0.2.5
IMAGE ACQUISITION EPU 3.6.0
CTF CORRECTION cryoSPARC 4.7
MODEL FITTING UCSF ChimeraX 1.9
MODEL FITTING ISOLDE 1.9
INITIAL EULER ASSIGNMENT cryoSPARC 4.7
FINAL EULER ASSIGNMENT cryoSPARC 4.7
CLASSIFICATION cryoSPARC 4.7
RECONSTRUCTION cryoSPARC 4.7
MODEL REFINEMENT Servalcat 0.4.99
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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