ELECTRON MICROSCOPY

Sample

Cryo-EM structure of the catalytic core of human telomerase at the elongation state of the repeat addition cycle

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 202390
Reported Resolution (Å) 3.2
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 48
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2400
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 81000
Calibrated Magnification 45872
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 4.0
IMAGE ACQUISITION EPU 2.13.0.3175REL
CTF CORRECTION CTFFIND 4
MODEL FITTING Coot 0.9.8.1
MODEL FITTING UCSF ChimeraX 1.5
MODEL REFINEMENT REFMAC 5.8
MODEL REFINEMENT Servalcat 0.4.70
INITIAL EULER ASSIGNMENT RELION 4.0
FINAL EULER ASSIGNMENT RELION 5.0
CLASSIFICATION RELION 5.0
RECONSTRUCTION RELION 5.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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