ELECTRON MICROSCOPY

Sample

Human mitochondrial 55S ribosome complex with tRNAs and COX1 nascent chain

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument HOMEMADE PLUNGER
Cryogen Name ETHANE
Sample Vitrification Details Manual blotting & plunge-freezing
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 70865
Reported Resolution (Å) 3.1
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target RSCC
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON III (4k x 4k)
Electron Dose (electrons/Å2) 35
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 200
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 0.01
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 59000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Gautomatch ?
IMAGE ACQUISITION EPU ?
CTF CORRECTION Gctf ?
MODEL FITTING Coot ?
MODEL FITTING UCSF ChimeraX ?
MODEL REFINEMENT PHENIX ?
MODEL REFINEMENT Coot ?
INITIAL EULER ASSIGNMENT RELION 5.0
FINAL EULER ASSIGNMENT RELION 5.0
CLASSIFICATION RELION 5.0
RECONSTRUCTION cryoSPARC ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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