ELECTRON MICROSCOPY

Sample

Antibody-antigen complex between V1WT_06 antibody FAB and Bat SARS-like coronavirus WIV1 Spike glycoprotein

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE-PROPANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 3514633
Reported Resolution (Å) 3.98
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 32.2
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1500
Maximum Defocus (nm) 3000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification 75000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION crYOLO ?
PARTICLE SELECTION Topaz ?
IMAGE ACQUISITION EPU ?
MODEL FITTING UCSF Chimera ?
MODEL REFINEMENT PHENIX 1.21.2_5419
INITIAL EULER ASSIGNMENT cryoSPARC 4.6.2
FINAL EULER ASSIGNMENT cryoSPARC 4.6.2
CLASSIFICATION cryoSPARC 4.6.2
RECONSTRUCTION cryoSPARC 4.6.2
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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