ELECTRON MICROSCOPY

Sample

VPS34-CII-BATS bound to membrane-attached RAB5A-GTP (C19S, C63S, Q79L) on a LUV membrane

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK I
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SUBTOMOGRAM AVERAGING
Number of Particles 8441
Reported Resolution (Å) 9.88
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target cross-correlation coefficient
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 2.99
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 2000
Maximum Defocus (nm) 5000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 81000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
VOLUME SELECTION crYOLO 1.9.9
VOLUME SELECTION RELION 5.0 beta4-commit-dfea29
MODEL REFINEMENT PHENIX 1.21rc1_5156:
CTF CORRECTION CTFFIND 4.1
CTF CORRECTION RELION 5.0 beta4-commit-dfea29
FINAL EULER ASSIGNMENT RELION 5.0 beta4-commit-dfea29
CLASSIFICATION RELION 5.0 beta4-commit-dfea29
RECONSTRUCTION RELION 5.0 beta4-commit-dfea29
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION CTF estimated with CTFFIND-4.1 (wrapper in Relion5), then corrected during 3D refinement and CTF tomo refine in Relion5.
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