ELECTRON MICROSCOPY

Sample

Ryanodine receptor type 1 in complex with FKBP12 in the sarcoplasmic reticulum membrane

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 27861
Reported Resolution (Å) 4
Resolution Method FSC 0.143 CUT-OFF
Other Details Focused map consists of parts refined to the resolutions 3.9, 5.1, 4.2 and 4.1 Angstrom.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 59.9
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 4000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 64000
Calibrated Magnification 64000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.6.0
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 4.6.0
MODEL FITTING UCSF ChimeraX ?
MODEL REFINEMENT ISOLDE ?
MODEL REFINEMENT PHENIX ?
INITIAL EULER ASSIGNMENT cryoSPARC 4.6.0
FINAL EULER ASSIGNMENT cryoSPARC 4.6.0
RECONSTRUCTION cryoSPARC 4.6.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
Feedback Form
Name
Email
Institute
Feedback