ELECTRON MICROSCOPY

Sample

Rhodospirillum rubrum encapsulated ferritin

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 4 uL of sample was applied to the grids, which were then blotted 100% humidity blot force 5 wait time 10 s blot time 3 seconds
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 272846
Reported Resolution (Å) 2.9
Resolution Method FSC 0.143 CUT-OFF
Other Details Final reconstruction performed by local refinement of aligned encapsulated ferritin decamers from custom symmetry expansion.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target Cross-correlation coefficient
Overall B Value 146.6
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 1600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.7
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC ?
MODEL FITTING UCSF ChimeraX 9.03
INITIAL EULER ASSIGNMENT cryoSPARC ?
FINAL EULER ASSIGNMENT cryoSPARC ?
CLASSIFICATION cryoSPARC ?
RECONSTRUCTION cryoSPARC 4.7
MODEL REFINEMENT PHENIX 1.21.2_5419
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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