ELECTRON MICROSCOPY

Sample

Rhodospirillum rubrum encapsulin

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details blot force 5, wait time 10 s, and blot time 3 seconds
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 639134
Reported Resolution (Å) 2
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol AB INITIO MODEL
Refinement Target Cross correlation
Overall B Value 70
Fitting Procedure ?
Details Real space refinement in phenix
Data Acquisition
Detector Type FEI FALCON III (4k x 4k)
Electron Dose (electrons/Å2) 42
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 1600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.7
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 4.7
MODEL FITTING UCSF ChimeraX 1.10
MODEL REFINEMENT PHENIX 1.21.2_5419
INITIAL EULER ASSIGNMENT cryoSPARC 4.7
FINAL EULER ASSIGNMENT cryoSPARC 4.7
CLASSIFICATION cryoSPARC 4.7
RECONSTRUCTION cryoSPARC 4.7
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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